Journal: PLOS One

Article Title: Effects and mechanisms of engineered exosomes pretreated with Scutellaria baicalensis Georgi on osteoporosis

doi: 10.1371/journal.pone.0333897

Figure Lengend Snippet: (A) RUNX2 immunofluorescence staining. (B) Western blotting of RUNX2. (C) Quantitative results of RUNX2 western blotting. (D) The transcriptional expression level of Sema3A. (E) Western blotting of Sema3A. (F) Quantitative results of Sema3A western blotting. Significance levels are indicated as follows: * for p < 0.05, ** for p < 0.01, *** for p < 0.001, and **** for p < 0.0001.

Article Snippet: Antibodies targeting RUNX2 and Sema3A were obtained by Proteintech (Wuhan, China).

Techniques: Immunofluorescence, Staining, Western Blot, Expressing

Journal: PLOS One

Article Title: Effects and mechanisms of engineered exosomes pretreated with Scutellaria baicalensis Georgi on osteoporosis

doi: 10.1371/journal.pone.0333897

Figure Lengend Snippet: (A) Volcano plot showing differentially expressed genes. (B) Heatmap illustrating expression patterns of Sema3A-related genes. (C) WB analysis confirming the elevated Sema3A levels in S-EXOs@BMSCs compared with EXOs@BMSCs. (D) Representative images and quantitative analysis demonstrating that inhibition of Sema3A attenuates the osteogenic effects of S-EXOs@BMSCs. Significance levels are indicated as follows: * for p < 0.05, ** for p < 0.01, *** for p < 0.001, and **** for p < 0.0001.

Article Snippet: Antibodies targeting RUNX2 and Sema3A were obtained by Proteintech (Wuhan, China).

Techniques: Expressing, Inhibition

Journal: PLOS One

Article Title: Effects and mechanisms of engineered exosomes pretreated with Scutellaria baicalensis Georgi on osteoporosis

doi: 10.1371/journal.pone.0333897

Figure Lengend Snippet: (A) Mouse femurs were analyzed using H&E and Masson’s trichrome staining techniques. (B) The fluorescence staining results of OCN, TRAP, and Sema3A triple co-staining in mouse femurs.

Article Snippet: Antibodies targeting RUNX2 and Sema3A were obtained by Proteintech (Wuhan, China).

Techniques: Staining, Fluorescence

Journal: Cell

Article Title: A mast cell receptor mediates post-stroke brain inflammation via a dural-brain axis

doi: 10.1016/j.cell.2025.06.045

Figure Lengend Snippet: (A) Representative flow cytometry gating of WT/ Mrgprb2 −/− brains 48h post-tMCAO. left , Live cells are gated using an immune marker (CD45) and myeloid marker (CD11b). right , Myeloid cells are gated using Ly6G and Ly6C to delineate CD45-high neutrophils and monocytes/macrophages, and CD45-low microglia. (B-D) (B) Absolute count of neutrophils, (C) monocytes/macrophages, and (D) CD11b-positive microglia in contralateral/stroke brain hemispheres of WT/ Mrgprb2 −/− mice 48h post-tMCAO (WT n =18, Mrgprb2 −/− n =16). (E) Representative immunofluorescence images of WT (left)/ Mrgprb2 −/− (right) right brain hemispheres 48h post-tMCAO. top row, CD45-positive immune cells. middle row, GFAP-positive activated astrocytes. Dotted white line separates infarcted tissue without GFAP and live, injured brain tissue with GFAP. bottom row, Merged channels with DAPI. Scale bar=50 μm. (F), left, CCL2 and right, CCL3 protein expression measured by ELISA in contralateral/stroke brain hemispheres of WT/ Mrgprb2 −/− mice (CCL2: WT n =6, Mrgprb2 −/− n =6, CCL3: WT n =5, Mrgprb2 −/− n =5). (G-H) (G) IL-6 and (H) neutrophil elastase protein expression measured by ELISA in contralateral/stroke brain hemispheres of WT/ Mrgprb2 −/− mice (G, WT n =5, Mrgprb2 −/− n =5, H, WT n =7, Mrgprb2 −/− n =7). (I) Representative immunofluorescence image of Mrgprb2 −/− mouse meninges 8 weeks after engraftment with Mrgprb2-tdT mast cells. left column, tdT denotes engrafted cells. middle column, Avidin denotes all mast cells. right column, Merged channels with DAPI. Scale bar=50 μm. (J) Mast cell count in meninges of Mrgprb2 −/− mice engrafted with saline, WT, or Mrgprb2 −/− mast cells, determined by Avidin-positive cells in the dura, using a 0.408mm 2 viewing frame (WT saline n =4, WT engraftment n =5, Mrgprb2 −/− saline n =4, Mrgprb2 −/− engraftment n =4). (K) Absolute count of neutrophils and monocytes/macrophages in contralateral/stroke brain hemispheres of WT/ Mrgprb2 −/− mast cell engrafted mice (WT n =16, Mrgprb2 −/− n =18). (L). left, Representative T2-weighted MRIs, and right , stroke volume of WT/ Mrgprb2 −/− MC-engrafted Mrgprb2 −/− mice 48h post-tMCAO (WT n =9, Mrgprb2 −/− n =8). Statistical analyses: Kruskal-Wallis test (B and G), two-way ANOVA with Sidak’s multiple comparisons test (C-D, F, H, and J-K), and two-sided Student’s t-test (L). Statistical test for C, D, and K was performed on log-transformed data to adjust for non-normality. Bar graphs indicate mean ± SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: Mouse Semaphorin 3A ELISA Kit , Cusabio , Cat#: CSB-EL020980MO.

Techniques: Flow Cytometry, Marker, Immunofluorescence, Expressing, Enzyme-linked Immunosorbent Assay, Avidin-Biotin Assay, Cell Counting, Saline, Transformation Assay